| Zak, E., Norling, B., Maitra, R., Huang, F., Andersson, B. and Pakrasi, H.B. (2001) | |
| The initial steps of biogenesis of cyanobacterial photosystems occur in plasma membranes. PROC NATL ACAD SCI USA; in press.
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| Regel, R., Ivleva, N.B., Zer, H., Meurer, J., Shestakov, S.V., Herrmann,R.G., Pakrasi, H.B. and Ohad, I. (2001) | |
| Deregulation of electron flow
within Photosystem II in the absence of the PsbJ protein. J BIOL CHEM;
in press. |
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| Inagaki, N., Maitra, R., Satoh, K. and Pakrasi, H. B. (2001) | |
| Amino acid residues that are critical for in vivo catalytic activity of CtpA, the carboxyl-terminal processing protease for the D1 Protein of Photosystem
II. J BIOL CHEM 276: 30099-30105. |
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| Pakrasi, H.B., Ogawa, T. and Bhattacharyya-Pakrasi, M. (2001) | |
| Transport of
metals: a key process in oxygenic photosynthesis. In Regulatory Aspects
of Photosynthesis (Aro, E.-M. and Andersson, B.A., eds.), pp. 253-264,
Kluwer Academic Publ., Dordrecht, The Netherlands. |
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| Ng, W.-O. and Pakrasi, H.B. (2001) | |
| DNA photolyase homologues are the major
UV resistance factors in Synechocystis sp. PCC 6803. MOL GEN GENET
264: 924-930. |
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| Ng, W.-O., Zentella, R., Wang, Y., Taylor, J.-S. A., and Pakrasi, H.B. (2000) | |
| phrA, the major photoreactivating factor in the cyanobacterium Synechocystis sp. strain PCC 6803 codes for a cyclobutane-pyrimidine-dimer-specific DNA photolyase. ARCH MICROBIOL 173: 412-417. |
Ohkawa, H., Pakrasi, H.B. and Ogawa, T. (2000) |
| Two types of functionally distinct NAD(P)H dehydrogenases in Synechocystis sp. strain PCC6803. J BIOL CHEM 275: 31630 - 31634. |
|
| Ivleva, N.B., Shestakov, S.V. and Pakrasi H.B. (2000) | |
| The carboxyl-terminal extension of the precursor D1 protein of Photosystem II is required for optimal photosynthetic performance of the cyanobacterium Synechocystis sp. PCC 6803. PLANT PHYSIOL 124: 1403-1411. |
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| Zak, E. and Pakrasi, H.B. (2000) | |
| The BtpA protein stabilizes the reaction center proteins of Photosystem I
the cyanobacterium Synechocystis sp. PCC 6803. PLANT PHYSIOL 123: 215-222. |
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| Cho, Y.S., Pakrasi, H.B. and Whitmarsh, J. (2000) | |
| Cytochrome cM from Synechocystis 6803: detection in cells, expression in Escherichia coli, purification and physical characterization. EUR J BIOCHEM 267: 1067-1074. |
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| Meetam M, Keren N, Ohad I, Pakrasi HB | |
| The PsbY protein is not essential for oxygenic photosynthesis in the cyanobacterium Synechocystis sp PCC 6803 A tetra-manganese cluster in the photosystem II (PSII) pigment-protein complex plays a critical role in the photosynthetic oxygen evolution process. PsbY, a small membrane-spanning polypeptide, has recently been suggested to provide a ligand for manganese in PSII (A.E. Gau, H.H. Thole, A. Sokolenko, L. Altschmied, R.G. Herrmann, E.K. Pistorius [1998] Mol Gen Genet 260: 56-68). We have constructed a mutant strain of the cyanobacterium Synechocystis sp. PCC 6803 with an inactivated psbY gene (sml0007). Southern-blot and polymerase chain reaction analysis showed that the mutant had completely segregated. However, the Delta psbY mutant cells grew normally under photoautotrophic conditions. Moreover, growth of the wild-type and mutant cells were similar under highlight photoinhibition conditions, as well as in media without any added manganese, calcium, or chloride, three required inorganic cofactors for the oxygen-evolving complex of PSII. Analysis of steady-state and flash-induced oxygen evolution, fluorescence induction, and decay kinetics, and thermoluminescence profiles demonstrated that the Delta psbY mutant cells have normal photosynthetic activities. We conclude that the PsbY protein in Synechocystis 6803 is not essential for oxygenic photosynthesis and does not provide an important binding site for manganese in the oxygen-evolving complex of PSII. PLANT PHYSIOL 121: (4) 1267-1272 DEC 1999 |
|
| Bartsevich VV, Pakrasi HB | |
| Membrane topology of MntB, the transmembrane protein component of an ABC transporter system for manganese in the cyanobacterium Synechocystis sp strain PCC 6803
J BACTERIOL 181: (11) 3591-3593 JUN 1999 |
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| Korshunova YO, Eide D, Clark WG, Guerinot ML, Pakrasi HB | |
| The IRT1 protein from Arabidopsis thaliana is a metal transporter with a broad substrate range
PLANT MOL BIOL 40: (1) 37-44 MAY 1999 |
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| Zak E, Norling B, Andersson B, Pakrasi HB | |
| Subcellular localization of the BtpA protein in the cyanobacterium Synechocystis sp. PCC 6803
PEUR J BIOCHEM 261: (1) 311-316 APR 1999 |
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| Norling B, Zak E, Andersson B, Pakrasi H | |
| 2D-isolation of pure plasma and thylakoid membranes from the cyanobacterium Synechocystis sp. PCC 6803
FEBS LETT 436: (2) 189-192 OCT 2 1998 |
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| McNamara VP, Sutterwala FS, Pakrasi HB, Whitmarsh J | |
| Structural model of cytochrome b(559) in photosystem II based on a mutant with genetically fused subunits
P NATL ACAD SCI USA 94: (25) 14173-14178 DEC 9 1997 |
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| Karnauchov I, Herrmann RG, Pakrasi HB, Klosgen RB | |
| Transport of CtpA protein from the cyanobacterium Synechocystis 6803 across the thylakoid membrane in chloroplasts
EUR J BIOCHEM 249: (2) 497-504 OCT 15 1997 |
|
| Bartsevich VV, Pakrasi HB | |
| Molecular identification of a novel protein that regulates biogenesis of photosystem .1. A membrane protein complex
J BIOL CHEM 272: (10) 6382-6387 MAR 7 1997 |
|
| Bartsevich VV, Pakrasi HB | |
| Manganese transport in the cyanobacterium Synechocystis sp PCC 6803
J BIOL CHEM 271: (42) 26057-26061 OCT 18 1996 |
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| Oelmuller R, Herrmann RG, Pakrasi HB | |
| Molecular studies of CtpA, the carboxyl-terminal processing protease for the D1 protein of the photosystem II reaction center in higher plants
J BIOL CHEM 271: (36) 21848-21852 SEP 6 1996 |
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| Mannan RM, He WZ, Metzger SU, Whitmarsh J, Malkin R, Pakrasi HB | |
| Active photosynthesis in cyanobacterial mutants with directed modifications in the ligands for two iron-sulfur clusters in the PsaC protein of photosystem I
EMBO J 15: (8) 1826-1833 APR 15 1996 |
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| Whitmarsh, J. and Pakrasi, H.B. | |
| Form and Function of cytochrome b559.
Advances in Photosynthesis, vol. 4, Oxygenic Photosynthesis: The Light Reactions, eds. Ort, D.R and Yocum, C.F., pp. 249-264. Kluwer Academic Press, The Netherlands 1996 |
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