LevinLab

Genetics and Cloning

• Codon Bias in B. subtilis
• Chemical Competence for E. coli
• Competence and Transforming into B. subtilis
• Chromosome Prep, small scale, for B. subtilis
• Chromosome Prep, large scale, B. subtilis
• Chromosomal Prep for B. subtilis, by Amy Buscher
• P1 vir Phage Transduction in E. coli, by Brad Weart
• P1 vir Phage Transduction in E. coli, Brian San Francisco's adaptation
• Plaque Assay for Phage in E. coli

Proteins and Biochemistry

• Western Blot
• PAGE
• SDS-PAGE Gel Recipes
• FtsZ Protein Prep
• TLC Protocol, by Brad Weart
• TLC Protocol, Mimi's Fabulous
• Use of the Spectrofluorimeter
• Full Induction in a T7-polymerase-containing strain
• Mini Inducion in a T7-polymerase-containing strain
• Ponceau-S Stain

Microscopy

• Poly-L-Lysine Slide Mounting
• Staining with FM4-64 Membrane Stain
• Ethanol Fixation and DAPI Staining
• Gluteraldehyde/Paraformaldehyde Fixation
• IFM
• Methanol Fix a la Esther
• Using Live Membrane Stain

Antibodies

• Affinity Purification of Antibodies
• Storage and Reuse of Antibodies (for Westerns)
• Storage of Antibodies
• Storage of HRP-conjugaed antibodies

General B. subtilis Biology

• Sporulation Assay for B. subtilis
• Maximum Centrifuge RPMs for Various Vessels